Cytokinin response factors regulate PIN-FORMED auxin transporters Academic Article uri icon

abstract

  • Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.

authors

  • Šimášková, M
  • O'Brien, JA
  • Khan, M
  • Van Noorden, G
  • Ötvös, K
  • Vieten, A
  • De Clercq, I
  • Van Haperen, JMA
  • Cuesta, C
  • Hoyerová, K
  • Vanneste, S
  • Marhavý, P
  • Wabnik, K
  • Van Breusegem, F
  • Nowack, M
  • Murphy, A
  • Friml, J
  • Weijers, D
  • Beeckman, T
  • Benková, E

publication date

  • 2015