Targeting Antigen to Mouse Dendritic Cells via Clec9A Induces Potent CD4 T Cell Responses Biased toward a Follicular Helper Phenotype Academic Article uri icon

abstract

  • Three surface molecules of mouse CD8(+) dendritic cells (DCs), also found on the equivalent human DC subpopulation, were compared as targets for Ab-mediated delivery of Ags, a developing strategy for vaccination. For the production of cytotoxic T cells, DEC-205 and Clec9A, but not Clec12A, were effective targets, although only in the presence of adjuvants. For Ab production, however, Clec9A excelled as a target, even in the absence of adjuvant. Potent humoral immunity was a result of the highly specific expression of Clec9A on DCs, which allowed longer residence of targeting Abs in the bloodstream, prolonged DC Ag presentation, and extended CD4 T cell proliferation, all of which drove highly efficient development of follicular helper T cells. Because Clec9A shows a similar expression pattern on human DCs, it has particular promise as a target for vaccines of human application.

authors

  • Lahoud, Mireille H
  • Ahmet, Fatma
  • Kitsoulis, Susie
  • Wan, Soo San
  • Vremec, David
  • Lee, Chin-Nien
  • Phipson, Belinda
  • Shi, Wei
  • Smyth, Gordon K
  • Lew, Andrew M
  • Kato, Yu
  • Mueller, Scott N
  • Davey, Gayle M
  • Heath, William R
  • Shortman, Ken
  • Caminschi, Irina

publication date

  • July 15, 2011

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