Cleavage and activation of proteinase-activated receptor-2 on human neutrophils by gingipain-R from Porphyromonas gingivalis Academic Article uri icon

abstract

  • Gingipain-R, the major arginine-specific proteinase from Porphyromonas gingivalis, a causative agent of adult periodontal disease, was found to cleave a model peptide representing the cleavage site of proteinase-activated receptor-2 (PAR-2), a G-protein-coupled receptor found on the surface of neutrophils. The bacterial proteinase was also shown to induce an increase in the intracellular calcium concentration of enzyme-treated neutrophils, most probably due to PAR-2 activation. This response by neutrophils to gingipain-R may be a mechanism for the development of inflammation associated with periodontal disease.

authors

  • Lourbakos, Afrodite
  • Chinni, Carla
  • Thompson, Philip
  • Potempa, Jan
  • Travis, James
  • Mackie, Eleanor J
  • Pike, Robert N

publication date

  • September 11, 1998