Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate Academic Article uri icon

abstract

  • A 6-h procedure for the isolation of bovine cathepsin D is described. The procedure involves essentially only two steps; three-phase partitioning in t-butanol/water/ammonium sulfate followed by affinity chromatography on pepstatin-agarose. The major advantage of this new method over previous methods is the greatly reduced time required to obtain comparably pure cathepsin D.

publication date

  • July 1989