The aims of this experiment were to determine whether there are differences between the sexes in the three-dimensional angular rotations of the lumbo-pelvic-hip complex during running and, if such differences exist, to establish whether factors other than sex can explain the observed differences. A cohort of 44 non-injured runners (22 males, 22 females) who usually ran more than 20 km per week were voluntarily recruited. All trials were conducted on a treadmill at a running speed of 4.0 m x s(-1). Reflective markers were placed over specific anatomical landmarks of the thoraco-lumbar spine, pelvis and femur. Data were captured using a VICON motion analysis system. Females tended to display a greater peak-to-peak oscillation for most of the angular rotations. An offset was the main difference between the male and female group mean waveforms for pelvis anterior-posterior tilt. Forward stepwise regression analysis revealed that sex was the most common variable related to the amplitudes of the angular rotations. Given these results, sport scientists conducting future biomechanical studies using angular data to test hypotheses are advised to be extremely cautious about averaging across male and female participants.