From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography Academic Article uri icon

abstract

  • Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RCvir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RCvir serial femtosecond crystallography (SFX) structure from 3.3-Å to 2.4-Å resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.

authors

  • Dods, Robert
  • Båth, Petra
  • Arnlund, David
  • Beyerlein, Kenneth R
  • Nelson, Garrett
  • Liang, Mengling
  • Harimoorthy, Rajiv
  • Berntsen, Peter
  • Malmerberg, Erik
  • Johansson, Linda
  • Andersson, Rebecka
  • Bosman, Robert
  • Carbajo, Sergio
  • Claesson, Elin
  • Conrad, Chelsie E
  • Dahl, Peter
  • Hammarin, Greger
  • Hunter, Mark S
  • Li, Chufeng
  • Lisova, Stella
  • Milathianaki, Despina
  • Robinson, Joseph
  • Safari, Cecilia
  • Sharma, Amit
  • Williams, Garth
  • Wickstrand, Cecilia
  • Yefanov, Oleksandr
  • Davidsson, Jan
  • DePonte, Daniel P
  • Barty, Anton
  • Brändén, Gisela
  • Neutze, Richard

publication date

  • 2017