Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody Academic Article uri icon


  • Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed using immunohistochemical staining. Here we report on the use of Fourier transform infrared (FTIR) hyperspectral imaging, a method widely used in two-dimensional analysis of chemical components, to study HLA class I expression in tissue. Two-dimensional cluster colour images derived from unsupervised hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections were compared with consecutive sections that were immunohistochemically stained for class I expression. HLA-class-I-positive and -negative areas were differentiated by FTIR cluster images in all eight melanoma sections investigated without the need for antibody attachment. FTIR imaging enables the distinction of HLA-class-I-positive from class-I-negative areas in melanoma. This method is accurate, rapid and cost-effective.


  • Chew, SF
  • Wood, BR
  • Kanaan, C
  • Browning, J
  • MacGregor, D
  • Davis, ID
  • Cebon, J
  • Tait, BD
  • McNaughton, D

publication date

  • April 2007