Cancer testis antigens expression in mesothelioma: role of DNA methylation and bioimmunotherapeutic implications Academic Article uri icon

abstract

  • Recent evidences suggest that malignant mesothelioma may be sensitive to immunotherapy; however, little is known about malignant mesothelioma-associated tumour antigens. Focusing on cancer/testis antigens, the expression of well-characterised immunogenic tumour-associated antigens was investigated in malignant mesothelioma cells. At variance with MAGE-4 and NY-ESO-1, malignant mesothelioma cells frequently expressed MAGE-1, -2 and -3, GAGE 1-2, GAGE 1-6, SSX-2 and SSX 1-5, and distinct malignant mesothelioma cells concomitantly expressed at least four cancer/testis antigens. Additionally, the tumour-associated antigens RAGE-1 was expressed at high levels in both benign and malignant mesothelial cells. Lastly, treatment with the DNA hypomethylating agent 5-aza-2'-deoxycytidine induced and up-regulated the expression of the cancer/testis antigen examined in malignant mesothelioma cells. Overall, these findings strongly suggest that cancer/testis antigens-based immunotherapy may represent a suitable therapeutic approach to malignant mesothelioma, and foresee the clinical use of 5-aza-2'-deoxycytidine to design new chemo-immunotherapeutic strategies in malignant mesothelioma patients.

authors

  • Sigalotti, L
  • Coral, S
  • Altomonte, M
  • Natali, L
  • Gaudino, G
  • Cacciotti, P
  • Libener, R
  • Colizzi, F
  • Vianale, G
  • Martini, F
  • Tognon, M
  • Jungbluth, A
  • Cebon, J
  • Maraskovsky, E
  • Mutti, L
  • Maio, M

publication date

  • March 2002