A Dominant CD4+ T-Cell Response to Helicobacter pylori Reduces Risk for Gastric Disease in Humans Academic Article uri icon

abstract

  • BACKGROUND & AIMS: Immunodominance is an important feature of antiviral, antitumor, and antibacterial cellular immune responses, but it is not well demonstrated in the immune responses against Helicobacter pylori. Antigen-specific CD4(+) T cells protect mice against infection with H pylori. We investigated the immunodominant CD4(+) T-cell response to neuraminyllactose-binding hemagglutinin (HpaA), which is a conserved, H pylori-specific colonization factor that is being investigated as an antigen for vaccination strategies. METHODS: HpaA-specific CD4(+) T cells were expanded with autologous peripheral blood mononuclear cells that had been incubated with recombinant HpaA and characterized using overlapping synthetic peptides. We compared the percentage of CD4(+) T cells with specificity for HpaA(88-100), restricted to HLA-DRB1*1501, among 59 H pylori-infected subjects with different gastric diseases. RESULTS: We identified and characterized several immunodominant CD4(+) T-cell epitopes derived from HpaA. The immunodominant CD4(+) T-cell responses specific to HpaA(88-100) were observed in most H pylori-infected individuals who expressed HLA-DRB1*1501 and were significantly more abundant in patients with less severe diseases (P < .05). CONCLUSIONS: The HLA-DRB1*1501-restricted immunodominant CD4(+) T-cell response to HpaA(88-100) is associated with reduced risk of severe gastric diseases. Further study of these and other immunodominant CD4(+) T-cell responses to H pylori will provide insight into mechanisms of protective immunity and aid in vaccine design.

authors

  • Chen, Li
  • Li, Bin
  • Yang, Wu–Chen
  • He, Jia–Lin
  • Li, Ning–Yi
  • Hu, Jian
  • He, Ya–Fei
  • Yu, Shu
  • Zhao, Zhuo
  • Luo, Ping
  • Zhang, Jin–Yong
  • Li, Hai–Bo
  • Zeng, Ming
  • Lu, Dong–Shui
  • Li, Bo–Sheng
  • Guo, Hong
  • Yang, Shi–Ming
  • Guo, Gang
  • Mao, Xu–Hu
  • Chen, Weisan
  • Wu, Chao
  • Zou, Quan–Ming

publication date

  • March 2013