To investigate the effect of competitive football match congestion on hamstring strength and lower limb flexibility.Repeated measures.Elite male youth football.Fifteen male elite youth football players from the national football association centre of excellence were included (age = 15.81 ±0.65 years, height = 171.95 ±6.89 cm, weight = 65.93 ±7.53 kg).Hamstring strength and pain, ankle dorsiflexion, hip extension, knee extension and flexion range of motion.Hamstring strength was highest at baseline and significantly reduced at 24 (p = 0.001, mean difference -0.19 Nm/Kg, CI95 -0.28, -0.1) and 48 h post-match 1 (p = 0.002, mean difference -0.16 Nm/Kg, CI95 -0.25, -0.07). Strength recovered by match day 2 before significantly reducing again 24 h post-match 2 (p = 0.012, mean difference -0.17 Nm/Kg, CI95 -0.29, -0.04). Pain was lowest at baseline and increased in the post-match periods (p < 0.05) with standardised effect sizes ranging from 0.07 to 0.42. Passive knee flexion range decreased post-match (p < 0.01) with mean differences of 1.5°-2.7°. The other flexibility measures remained unaffected by match play.Isometric hamstring strength and pain can be considered for inclusion in-season to monitor player's post-match hamstring recovery characteristics during congested match fixtures.