A micro-scale method for determining relative metal-binding affinities of proteins Academic Article uri icon


  • This article describes a quick and easy method for determining relative binding affinities between proteins and metal ions. The method is based on separating unbound metal ions from metal ions bound to protein by ultrafiltration using microcentrifuge ultrafiltration units. Bovine serum albumin (BSA) was used as the test protein and the relative affinity towards divalent metal ions was found to be Cu2+ > Zn2+ > Cd2+ > Pb2+ > Ni2+ > Co2+, which corresponds to the relative orders reported in the literature.

publication date

  • December 1997