Genome Sequences and Characterization of the Related Gordonia Phages GTE5 and GRU1 and Their Use as Potential Biocontrol Agents Academic Article uri icon


  • ABSTRACTActivated sludge plants suffer frequently from the operational problem of stable foam formation on aerobic reactor surfaces, which can be difficult to prevent. Many foams are stabilized by mycolic acid-containingActinobacteria, the mycolata. Thein situbiocontrol of foaming using phages is an attractive strategy. We describe two polyvalent phages, GTE5 and GRU1, targetingGordonia terraeandGordonia rubrupertincta, respectively, isolated from activated sludge. Phage GRU1 also propagates onNocardia nova. Both phages belong to the familySiphoviridaeand have similar-size icosahedral heads that encapsulate double-stranded DNA genomes (∼65 kb). Their genome sequences are similar to each other but markedly different from those of other sequenced phages. Both are arranged in a modular fashion. These phages can reduce or eliminate foam formation by their host cells under laboratory conditions.

publication date

  • January 1, 2012