Hip and groin pain are common problems in Australian football. Although indigenous (I) players are at greater risk of soft tissue injury than their non-indigenous (non-I) counterparts, Aboriginal descent has not previously been identified as a risk factor for hip and groin injury. The aim of this study was to investigate if hip and groin screening tests would demonstrate differences between indigenous and non-indigenous junior elite AF players.Cross-sectional study.Two hundred and seventy elite junior Australian football players were screened using five hip and groin musculoskeletal tests.Thirty-three players (12%) were indigenous. Differences were demonstrated between the two groups for right prone hip internal rotation (I X = 27.60 ± 9.16, non-I X = 33.39 ± 8.88, p < 0.001) and left prone hip internal rotation (I X = 25.83 ± 10.25, non-I X = 31.36 ± 8.75, p < 0.001), pressure on squeeze test with knees at 90° (I X = 165.71 ± 40.32, non-I X = 188.17 ± 62.32, p = 0.001) and pressure on squeeze tests with knees at 0° (I X = 172.57 ± 35.98, non-I X = 202.57 ± 49.14, p = 0.049), and pain provocation during squeeze test with knees at 90° (I X = 3.19 ± 2.26, non-I X = 1.03 ± 1.78, p > 0.001).The indigenous players displayed less range of passive hip internal rotation with the hip in neutral, reduced adductor squeeze force and higher levels of groin pain with the squeeze test at 90°. The differences observed between indigenous and non-indigenous players suggest indigenous players are at greater risk of hip and groin injuries in Australian football.