IL-5-Deficient Mice Have a Developmental Defect in CD5+ B-1 Cells and Lack Eosinophilia but Have Normal Antibody and Cytotoxic T Cell Responses Academic Article uri icon

abstract

  • Mice deficient in interleukin-5 (IL-5-/- mice) were generated by gene targeting in embryonal stem cells. Contrary to previous studies, no obligatory role for IL-5 was demonstrated in the regulation of conventional B (B-2) cells, in normal T cell-dependent antibody responses or in cytotoxic T cell development. However, CD5+ B cells (B-1 cells) in the peritoneal cavity were reduced by 50%-80% in 2-week-old IL-5-/- mice, returning to normal by 6-8 weeks of age. The IL-5-/- mice did not develop blood and tissue eosinophilia when infected with the helminth Mesocestoides corti, but basal levels of eosinophils with normal morphology were produced in the absence of IL-5. IL-5 deficiency did not affect the worm burden of infected mice, indicating that increased eosinophils do not play a significant role in the host defence in this parasite model.

authors

  • Kopf, Manfred
  • Brombacher, Frank
  • Hodgkin, Philip D
  • Ramsay, Alistair J
  • Milbourne, Elizabeth A
  • Dai, Wen J
  • Ovington, Karen S
  • Behm, Carolyn A
  • Köhler, Georges
  • Young, Ian G
  • Matthaei, Klaus I

publication date

  • January 1996