Chromosome 7-Centric Analysis of Proteomics Data from a Panel of Human Colon Carcinoma Cell Lines Academic Article uri icon

abstract

  • In this manuscript, we describe a shotgun proteomics approach for a comprehensive proteomic analysis of samples including total lysates, membrane, secretome, and exosome fractions from a panel of colorectal cancer cell lines. We will present an analysis of our proteomics data in two alternative formats. First we will discuss a traditional analysis of our data, in which we identify a number of cancer-associated proteins using various proteomic data analysis tools. In a second approach, we use a chromosome format to organize the proteomic data on chromosome 7, allowing the identification of clusters of cancer-associated genes with boundaries defined by physical proximity on different chromosomes.

authors

  • Fanayan, Susan
  • Smith, Joshua T
  • Sethi, Manveen K
  • Cantor, David
  • Goode, Robert
  • Simpson, Richard J
  • Baker, Mark S
  • Hancock, William S
  • Nice, Edouard

publication date

  • January 4, 2013