Characterization of bovine heparin-binding neurotrophic factor (HBNF): Assignment of disulfide bonds Academic Article uri icon

abstract

  • The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.

authors

  • Fabri, L
  • Nice, EC
  • Ward, LD
  • Maruta, H
  • Burgess, AW
  • Simpson, RJ

publication date

  • January 1, 1992