Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent Academic Article uri icon


  • INTRODUCTIONThis protocol describes a method for quantitating the amount of free thiols and disulfide bond linkages in proteins. It uses the sulfhydryl reagent 5,5'-dithiobis (2-nitrobenzoic acid), or DTNB, which is also referred to as Ellman's reagent.

publication date

  • September 1, 2008