The amino-terminal sequence of the 85–90K nonhormone binding component of the molybdate-stabilized estradiol receptor from calf uterus Academic Article uri icon

abstract

  • The first six N-terminal amino acid residues of the 85-90K non-estrogen binding component of the calf uterine, molybdate-stabilized estradiol receptor have been determined by Edman degradation. After affinity chromatography of the stabilized receptor oligomer, the 85-90K unit was purified to homogeneity by preparative gel electrophoresis using electroelution for protein recovery. Inverse-gradient high performance liquid chromatography provided the 85-90K protein suitable for amino-terminal sequence analysis.

authors

  • Ratajczak, Thomas
  • Comber, Mark
  • Moir, Robert
  • Hähnel, Roland
  • Grego, B
  • Rubira, MR
  • Simpson, RJ

publication date

  • February 1987