Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast. Academic Article uri icon

abstract

  • We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

authors

  • Singh, MB
  • Hough, T
  • Theerakulpisut, P
  • Avjioglu, A
  • Davies, S
  • Smith, PM
  • Taylor, P
  • Simpson, RJ
  • Ward, LD
  • McCluskey, J

publication date

  • February 15, 1991