High-performance liquid chromatography of amino acids, peptides and proteins Academic Article uri icon

abstract

  • Procedures for the purification of native and phosphorylated human growth hormone (hGH), S-carboxymethylated hGH, and hGH tryptic peptides, based exclusively on reversed-phase chromatography have been developed. Combinations of several volatile ion-pairing systems with small- and large-pore n-alkylsilicas were exploited in a general strategy, which allowed high recoveries of various hGH-related polypeptides from enzymatic incubations, as well as rapid desalting of samples following chemical modifications of the native protein, such as reductive alkylation in 6 M guanidine hydrochloride. The influence of the elution conditions on retention behaviour of phosphorylated hGH and its tryptic peptides in reversed-phase high-performance liquid chromatography is discussed.

authors

  • Grego, B
  • Baldwin, GS
  • Knessel, JA
  • Simpson, RJ
  • Morgan, FJ
  • Hearn, MTW

publication date

  • August 1984