ISOLATION, AMINO ACID SEQUENCE AND ACTION OF GUINEA-PIG ACTH ON ALDOSTERONE PRODUCTION BY GLOMERULOSA CELLS Academic Article uri icon

abstract

  • Though minor sequence differences between-species have been reported for adrenocorticotrophin, ACTH(1-39), the steroidogenic moiety ACTH(1-24) has appeared invariant in mammals. We here report the isolation, purification and amino acid sequencing of guinea-pig (GP) ACTH in which Pro24 is replaced by Ala24, and the demonstration that GP-ACTH stimulates aldosterone production to maximal levels well above those seen with human ACTH(1-39) or Synacthen, ACTH(1-24) amide, the synthetic ACTH fragment widely used for diagnostic and therapeutic purposes.

authors

  • Smith, AI
  • Wallace, CA
  • Moritz, RL
  • Simpson, RJ
  • Schmauk-White, LB
  • Woodcock, EA
  • Funder, JW

publication date

  • November 1, 1987