Small-Scale Extraction of Recombinant Proteins from Bacteria Academic Article uri icon

abstract

  • Bacteria are particularly convenient for producing recombinant proteins for purification purposes. To monitor induction as well as the levels of recombinant protein expression, it is important to have a rapid, simple method for estimating bacterial protein expression. This protocol describes the preparation of small-scale bacterial extracts using cell lysis with 0.5% Triton X-100.

publication date

  • September 1, 2010