MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins Academic Article uri icon

abstract

  • Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNA(Glu), however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity.

authors

  • Davies, Stefan MK
  • Lopez Sanchez, Maria IG
  • Narsai, Reena
  • Shearwood, Anne-Marie J
  • Razif, Muhammad FM
  • Small, Ian D
  • Whelan, James
  • Rackham, Oliver
  • Filipovska, Aleksandra

publication date

  • October 19, 2012