Megahertz serial crystallography. Academic Article uri icon

abstract

  • The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

authors

  • Wiedorn, Max O
  • Oberthür, Dominik
  • Bean, Richard
  • Schubert, Robin
  • Werner, Nadine
  • Abbey, Brian
  • Aepfelbacher, Martin
  • Adriano, Luigi
  • Allahgholi, Aschkan
  • Al-Qudami, Nasser
  • Andreasson, Jakob
  • Aplin, Steve
  • Awel, Salah
  • Ayyer, Kartik
  • Bajt, Saša
  • Barák, Imrich
  • Bari, Sadia
  • Bielecki, Johan
  • Botha, Sabine
  • Boukhelef, Djelloul
  • Brehm, Wolfgang
  • Brockhauser, Sandor
  • Cheviakov, Igor
  • Coleman, Matthew A
  • Cruz-Mazo, Francisco
  • Danilevski, Cyril
  • Darmanin, Connie
  • Doak, R Bruce
  • Domaracky, Martin
  • Dörner, Katerina
  • Du, Yang
  • Fangohr, Hans
  • Fleckenstein, Holger
  • Frank, Matthias
  • Fromme, Petra
  • Gañán-Calvo, Alfonso M
  • Gevorkov, Yaroslav
  • Giewekemeyer, Klaus
  • Ginn, Helen Mary
  • Graafsma, Heinz
  • Graceffa, Rita
  • Greiffenberg, Dominic
  • Gumprecht, Lars
  • Göttlicher, Peter
  • Hajdu, Janos
  • Hauf, Steffen
  • Heymann, Michael
  • Holmes, Susannah
  • Horke, Daniel A
  • Hunter, Mark S
  • Imlau, Siegfried
  • Kaukher, Alexander
  • Kim, Yoonhee
  • Klyuev, Alexander
  • Knoška, Juraj
  • Kobe, Bostjan
  • Kuhn, Manuela
  • Kupitz, Christopher
  • Küpper, Jochen
  • Lahey-Rudolph, Janine Mia
  • Laurus, Torsten
  • Le Cong, Karoline
  • Letrun, Romain
  • Xavier, P Lourdu
  • Maia, Luis
  • Maia, Filipe RNC
  • Mariani, Valerio
  • Messerschmidt, Marc
  • Metz, Markus
  • Mezza, Davide
  • Michelat, Thomas
  • Mills, Grant
  • Monteiro, Diana CF
  • Morgan, Andrew
  • Mühlig, Kerstin
  • Munke, Anna
  • Münnich, Astrid
  • Nette, Julia
  • Nugent, Keith A
  • Nuguid, Theresa
  • Orville, Allen M
  • Pandey, Suraj
  • Pena, Gisel
  • Villanueva-Perez, Pablo
  • Poehlsen, Jennifer
  • Previtali, Gianpietro
  • Redecke, Lars
  • Riekehr, Winnie Maria
  • Rohde, Holger
  • Round, Adam
  • Safenreiter, Tatiana
  • Sarrou, Iosifina
  • Sato, Tokushi
  • Schmidt, Marius
  • Schmitt, Bernd
  • Schönherr, Robert
  • Schulz, Joachim
  • Sellberg, Jonas A
  • Seibert, M Marvin
  • Seuring, Carolin
  • Shelby, Megan L
  • Shoeman, Robert L
  • Sikorski, Marcin
  • Silenzi, Alessandro
  • Stan, Claudiu A
  • Shi, Xintian
  • Stern, Stephan
  • Sztuk-Dambietz, Jola
  • Szuba, Janusz
  • Tolstikova, Aleksandra
  • Trebbin, Martin
  • Trunk, Ulrich
  • Vagovic, Patrik
  • Ve, Thomas
  • Weinhausen, Britta
  • White, Thomas A
  • Wrona, Krzysztof
  • Xu, Chen
  • Yefanov, Oleksandr
  • Zatsepin, Nadia
  • Zhang, Jiaguo
  • Perbandt, Markus
  • Mancuso, Adrian P
  • Betzel, Christian
  • Chapman, Henry
  • Barty, Anton

publication date

  • 2018