Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes Academic Article uri icon

abstract

  • The adaptor protein FADD/MORT1 is essential for apoptosis induced by 'death receptors', such as Fas (APO-1/CD95), mediating aggregation and autocatalytic activation of caspase-8. Perhaps surprisingly, FADD and caspase-8 are also critical for mitogen-induced proliferation of T lymphocytes. We generated novel monoclonal antibodies specific for mouse FADD and caspase-8 to investigate whether cellular responses, apoptosis or proliferation, might be explained by differences in post-translational modification and subcellular localisation of these proteins. During both apoptosis signalling and mitogenic activation, FADD and caspase-8 aggregated in multiprotein complexes and formed caps at the plasma membrane but they did not colocalise with lipid rafts. Interestingly, mitogenic stimulation, but not Fas ligation, induced a unique post-translational modification of FADD. These different modifications may determine whether FADD and caspase-8 induce cell death or proliferation.

authors

  • O'Reilly, LA
  • Divisekera, U
  • Newton, K
  • Scalzo, K
  • Kataoka, T
  • Puthalakath, H
  • Ito, M
  • Huang, DCS
  • Strasser, A

publication date

  • July 2004

has subject area