Dehydration Converts DsbG Crystal Diffraction from Low to High Resolution Academic Article uri icon

abstract

  • Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 A resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 A resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.

authors

  • Heras, Begoña
  • Edeling, Melissa A
  • Byriel, Karl A
  • Jones, Alun
  • Raina, Satish
  • Martin, Jennifer L

publication date

  • February 2003