Crystallization and preliminary diffraction analysis of a DsbA homologue fromWolbachia pipientis Academic Article uri icon

abstract

  • alpha-DsbA1 is one of two DsbA homologues encoded by the Gram-negative alpha-proteobacterium Wolbachia pipientis, an endosymbiont that can behave as a reproductive parasite in insects and as a mutualist in medically important filarial nematodes. The alpha-DsbA1 protein is thought to be important for the folding and secretion of Wolbachia proteins involved in the induction of reproductive distortions. Crystals of native and SeMet alpha-DsbA1 were grown by vapour diffusion and belong to the monoclinic space group C2, with unit-cell parameters a = 71.4, b = 49.5, c = 69.3 A, beta = 107.0 degrees and one molecule in the asymmetric unit (44% solvent content). X-ray data were recorded from native crystals to a resolution of 2.01 A using a copper anode and data from SeMet alpha-DsbA1 crystals were recorded to 2.45 A resolution using a chromium anode.

authors

  • Kurz, M
  • Iturbe-Ormaetxe, I
  • Jarrott, R
  • O'Neill, SL
  • Byriel, KA
  • Martin, JL
  • Heras, B

publication date

  • February 1, 2008