Cleavage at Met-X Bonds by Cyanogen Bromide Academic Article uri icon


  • INTRODUCTIONSelective cleavage of a protein by cyanogen bromide generates a small number of relatively large peptide fragments. This protocol describes protein cleavage with cyanogen bromide, using ~100-fold molar excess of cyanogen bromide over the methionine. The reaction is performed under mild acidic conditions (e.g., 70% formic acid), under nitrogen, and in the dark. All reagents are volatile and can be readily removed by lyophilization.

publication date

  • March 2007