As human demands for freshwater resources increase, there is growing concern for the long-term viability of native fish populations globally. Understanding the movements of fish, and how these are influenced by or respond to environmental changes, is a critical knowledge requirement for effective management; however, limited information on the ecological requirements of many fish species currently hinders our ability to sustainably manage fish populations. In this paper, we use four native Australian fish species with contrasting life histories as case studies to demonstrate how data derived from acoustic and radiotelemetry studies can be synthesised into conceptual diagrams to help scientists and managers develop targeted and effective conservation management strategies. Commonalities in the observed movement patterns were observed among the species despite their contrasting life histories, but there were also important differences that need to be recognised in the development of species-specific conservation management strategies. We conclude by discussing how such information has been or could be incorporated into conservation management actions, including the implementation of environmental flow releases and the identification of critical habitats.