Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences Academic Article uri icon

abstract

  • We report a high-quality draft of the genome sequence of the grey, short-tailed opossum (Monodelphis domestica). As the first metatherian ('marsupial') species to be sequenced, the opossum provides a unique perspective on the organization and evolution of mammalian genomes. Distinctive features of the opossum chromosomes provide support for recent theories about genome evolution and function, including a strong influence of biased gene conversion on nucleotide sequence composition, and a relationship between chromosomal characteristics and X chromosome inactivation. Comparison of opossum and eutherian genomes also reveals a sharp difference in evolutionary innovation between protein-coding and non-coding functional elements. True innovation in protein-coding genes seems to be relatively rare, with lineage-specific differences being largely due to diversification and rapid turnover in gene families involved in environmental interactions. In contrast, about 20% of eutherian conserved non-coding elements (CNEs) are recent inventions that postdate the divergence of Eutheria and Metatheria. A substantial proportion of these eutherian-specific CNEs arose from sequence inserted by transposable elements, pointing to transposons as a major creative force in the evolution of mammalian gene regulation.

authors

  • Mikkelsen, Tarjei S
  • Wakefield, Matthew J
  • Aken, Bronwen
  • Amemiya, Chris T
  • Chang, Jean L
  • Duke, Shannon
  • Garber, Manuel
  • Gentles, Andrew J
  • Goodstadt, Leo
  • Heger, Andreas
  • Jurka, Jerzy
  • Kamal, Michael
  • Mauceli, Evan
  • Searle, Stephen MJ
  • Sharpe, Ted
  • Baker, Michelle L
  • Batzer, Mark A
  • Benos, Panayiotis V
  • Belov, Katherine
  • Clamp, Michele
  • Cook, April
  • Cuff, James
  • Das, Radhika
  • Davidow, Lance
  • Deakin, Janine E
  • Fazzari, Melissa J
  • Glass, Jacob L
  • Grabherr, Manfred
  • Greally, John M
  • Gu, Wanjun
  • Hore, Timothy A
  • Huttley, Gavin A
  • Kleber, Michael
  • Jirtle, Randy L
  • Koina, Edda
  • Lee, Jeannie T
  • Mahony, Shaun
  • Marra, Marco A
  • Miller, Robert D
  • Nicholls, Robert D
  • Oda, Mayumi
  • Papenfuss, Anthony T
  • Parra, Zuly E
  • Pollock, David D
  • Ray, David A
  • Schein, Jacqueline E
  • Speed, Terence P
  • Thompson, Katherine
  • VandeBerg, John L
  • Wade, Claire M
  • Walker, Jerilyn A
  • Waters, Paul D
  • Webber, Caleb
  • Weidman, Jennifer R
  • Xie, Xiaohui
  • Zody, Michael C
  • Graves, Jennifer A Marshall
  • Ponting, Chris P
  • Breen, Matthew
  • Samollow, Paul B
  • Lander, Eric S
  • Lindblad-Toh, Kerstin

publication date

  • May 2007

published in