Lipidic cubic phase serial millisecond crystallography using synchrotron radiation Academic Article uri icon

abstract

  • Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.

authors

  • Nogly, P
  • James, D
  • Wang, D
  • White, TA
  • Zatsepin, N
  • Shilova, A
  • Nelson, G
  • Liu, H
  • Johansson, L
  • Heymann, M
  • Jaeger, K
  • Metz, M
  • Wickstrand, C
  • Wu, W
  • Båth, P
  • Berntsen, P
  • Oberthuer, D
  • Panneels, V
  • Cherezov, V
  • Chapman, H
  • Schertler, G
  • Neutze, R
  • Spence, J
  • Moraes, I
  • Burghammer, M
  • Standfuss, J
  • Weierstall, U

publication date

  • 2015

published in