Regulation of the glial glutamate transporter GLT-1 by glutamate and δ-opioid receptor stimulation Academic Article uri icon

abstract

  • The excitatory effect of presynaptically released glutamate is tightly regulated and terminated by high affinity sodium-dependent glutamate transporters. The regulation of the glial glutamate transporter GLT-1 is potentially important in synaptic modulation. Using astroglial cultures prepared from the rat cerebral cortex, we found that the delta-opioid receptor agonist [D-pen2,D-pen5]-enkephalin decreases and glutamate increases the expression of the GLT-1 transporter mRNA. Corresponding changes in the uptake kinetics were found after incubation for 48 h with the respective agonists when glial glutamate uptake was measured in primary astroglial cultures. The data suggest that long-term receptor activation induces alterations in glial glutamate uptake properties.

authors

  • Thorlin, Thorleif
  • Roginski, Raymond S
  • Choudhury, Kanakendu
  • Nilsson, Michael
  • Rönnbäck, Lars
  • Hansson, Elisabeth
  • Eriksson, Peter S

publication date

  • April 3, 1998

has subject area