OBJECTIVES: To investigate whether KALRN gene variation is associated with ischemic stroke (IS). DESIGN AND METHODS: Associations to overall IS and IS subtypes were investigated in SAHLSIS, which comprises 844 patients with IS and 668 controls. RESULTS: Associations between KALRN SNPs and overall IS and cardioembolic stroke were detected. Associations for overall IS were investigated in two additional Swedish samples, but could not be replicated. CONCLUSION: KALRN gene variation is not associated with overall IS.