A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies Academic Article uri icon

abstract

  • The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.

authors

  • Sharma, Amit
  • Berntsen, Peter
  • Harimoorthy, Rajiv
  • Appio, Roberto
  • Sjohamn, Jennie
  • Jarva, Michael
  • Björling, Alexander
  • Hammarin, Greger
  • Westenhoff, Sebastian
  • Brändén, Gisela
  • Neutze, Richard

publication date

  • 2019