Background:Injuries to the hamstring are relatively common in professional cricketers (as they are in many team sports) and have increased in incidence in the "T20 era" (introduction of 20-over matches) of cricket since 2006. Methods:This study analyzed incidence of hamstring injury in the various elite male match types over a 20-year period (1995-1996 to 2014-2015 seasons). Risk factors for hamstring strain were assessed using a multivariate logistic regression analysis technique. Results:There were 276 match time-loss hamstring injuries recorded over a 20-year period at the Australian state or national player level, of which 170 occurred in one of 40,145 player match sets. The overall rate of match onset rate was 22.5 hamstring injuries per 1000 team days. Fast bowling onset injuries were the highest subcategory at a rate of 10.9 injuries per 1000 team days, although batting onset injuries were particularly common in 50-over (one day) international matches. Significant risk factors in logistic regression analysis, in addition to hamstring injury history, were being a fast bowler relative risk (RR) 2.5 (95% confidence interval (CI): 1.3-4.5) and playing a match in Australia RR 2.3 (95%CI: 1.3-3.9). Conclusion:Fast bowlers suffer more hamstring injuries than other playing roles in cricket, particularly in First Class (multi-day) cricket. Batsmen are more likely to get injured in 50-over (one day) cricket. Playing in Australia (compared to overseas venues) leads to increased risk of hamstring injury.