Preparing samples for crystallization of Bcl-2 family complexes Chapter uri icon

Book Title

  • Programmed cell death: methods and protocols

abstract

  • High-resolution protein structures determined by X-ray crystallography or NMR have proven invaluable for deciphering the molecular mechanisms underlying the function of a vast range of proteins. Here, we describe methods to generate complexes of proteins belonging to the Bcl-2 family of proteins with either biological ligands or small molecule antagonists.

publication date

  • 2016