Alternative cross-priming through CCL17-CCR4-mediated attraction of CTLs toward NKT cell–licensed DCs Academic Article uri icon

abstract

  • Cross-priming allows dendritic cells (DCs) to induce cytotoxic T cell (CTL) responses to extracellular antigens. DCs require cognate 'licensing' for cross-priming, classically by helper T cells. Here we demonstrate an alternative mechanism for cognate licensing by natural killer T (NKT) cells recognizing microbial or synthetic glycolipid antigens. Such licensing caused cross-priming CD8alpha(+) DCs to produce the chemokine CCL17, which attracted naive CTLs expressing the chemokine receptor CCR4. In contrast, DCs licensed by helper T cells recruited CTLs using CCR5 ligands. Thus, depending on the type of antigen they encounter, DCs can be licensed for cross-priming by NKT cells or helper T cells and use at least two independent chemokine pathways to attract naive CTLs. Because these chemokines acted synergistically, this can potentially be exploited to improve vaccinations.

authors

  • Semmling, Verena
  • Lukacs-Kornek, Veronika
  • Thaiss, Christoph A
  • Quast, Thomas
  • Hochheiser, Katharina
  • Panzer, Ulf
  • Rossjohn, Jamie
  • Perlmutter, Patrick
  • Cao, Jia
  • Godfrey, Dale I
  • Savage, Paul B
  • Knolle, Percy A
  • Kolanus, Waldemar
  • Förster, Irmgard
  • Kurts, Christian

publication date

  • April 2010

has subject area