Developmental changes in the lateral diffusion of Leydig cell membranes measured by the FRAP method Academic Article uri icon

abstract

  • A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility of membrane lipids and proteins were discovered.

authors

  • Zlatanov, IV
  • Foley, M
  • Birmingham, J
  • Garland, PB

publication date

  • September 28, 1987