Single-cell transcriptomics identifies the adaptation of Scart1⁺ Vγ6⁺ T cells to skin residency as activated effector cells Academic Article uri icon

abstract

  • IL-17-producing γδ T cells express oligoclonal Vγ4+ and Vγ6+ TCRs, mainly develop in the prenatal thymus, and later persist as long-lived self-renewing cells in all kinds of tissues. However, their exchange between tissues and the mechanisms of their tissue-specific adaptation remain poorly understood. Here, single-cell RNA-seq profiling identifies IL-17-producing Vγ6+ T cells as a highly homogeneous Scart1+ population in contrast to their Scart2+ IL-17-producing Vγ4+ T cell counterparts. Parabiosis demonstrates that Vγ6+ T cells are fairly tissue resident in the thymus, peripheral lymph nodes, and skin. There, Scart1+ Vγ6+ T cells display tissue-specific gene expression signatures in the skin, characterized by steady-state production of the cytokines IL-17A and amphiregulin as well as by high expression of the anti-apoptotic Bcl2a1 protein family. Together, this study demonstrates how Scart1+ Vγ6+ T cells undergo tissue-specific functional adaptation to persist as effector cells in their skin habitat.

authors

  • Tan, L
  • Sandrock, I
  • Odak, I
  • Aizenbud, Y
  • Wilharm, A
  • Barros-Martins, J
  • Tabib, Y
  • Borchers, A
  • Amado, T
  • Gangoda, Lahira
  • Herold, MJ
  • Schmidt-Supprian, M
  • Kisielow, J
  • Silva-Santos, B
  • Koenecke, C
  • Hovav, AH
  • Krebs, C
  • Prinz, I
  • Ravens, S

publication date

  • 2019