Chemiluminescence Detector with a Serpentine Flow Cell Academic Article uri icon

abstract

  • We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available "BacTiter-Glo" reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell.

authors

  • Terry, Jessica M
  • Adcock, Jacqui L
  • Olson, Don C
  • Wolcott, Duane K
  • Schwanger, Cassie
  • Hill, Lauren A
  • Barnett, Neil W
  • Francis, Paul S

publication date

  • December 15, 2008