High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Academic Article uri icon

abstract

  • Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth (`pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A2A adenosine receptor (A2AAR) were determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.

authors

  • Martin-Garcia, Jose M
  • Zhu, Lan
  • Mendez, Derek
  • Lee, Ming-Yue
  • Chun, Eugene
  • Li, Chufeng
  • Hu, Hao
  • Subramanian, Ganesh
  • Kissick, David
  • Ogata, Craig
  • Henning, Robert
  • Ishchenko, Andrii
  • Dobson, Zachary
  • Zhang, Shangji
  • Weierstall, Uwe
  • Spence, John CH
  • Fromme, Petra
  • Zatsepin, Nadia A
  • Fischetti, Robert F
  • Cherezov, Vadim
  • Liu, Wei

publication date

  • May 1, 2019

published in