Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography Academic Article uri icon

abstract

  • Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

authors

  • Weierstall, Uwe
  • James, Daniel
  • Wang, Chong
  • White, Thomas A
  • Wang, Dingjie
  • Liu, Wei
  • Spence, John CH
  • Bruce Doak, R
  • Nelson, Garrett
  • Fromme, Petra
  • Fromme, Raimund
  • Grotjohann, Ingo
  • Kupitz, Christopher
  • Zatsepin, Nadia A
  • Liu, Haiguang
  • Basu, Shibom
  • Wacker, Daniel
  • Won Han, Gye
  • Katritch, Vsevolod
  • Boutet, Sébastien
  • Messerschmidt, Marc
  • Williams, Garth J
  • Koglin, Jason E
  • Marvin Seibert, M
  • Klinker, Markus
  • Gati, Cornelius
  • Shoeman, Robert L
  • Barty, Anton
  • Chapman, Henry N
  • Kirian, Richard A
  • Beyerlein, Kenneth R
  • Stevens, Raymond C
  • Li, Dianfan
  • Shah, Syed TA
  • Howe, Nicole
  • Caffrey, Martin
  • Cherezov, Vadim

publication date

  • 2014