Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9 Academic Article uri icon

abstract

  • We have generated rat monoclonal antibodies that specifically recognise caspase-2 from many species, including mouse, rat and humans. Using these antibodies, we have investigated caspase-2 expression, subcellular localisation and processing. We demonstrate that caspase-2 is expressed in most tissues and cell types. Cell fractionation and immunohistochemistry experiments show that caspase-2 is found in the nuclear and cytosolic fractions, including a significant portion present in the Golgi complex. We found that caspase-2 is processed in response to many apoptotic stimuli but experiments with caspase-2 deficient mice demonstrated that it is not required for apoptosis of thymocytes or dorsal root ganglia (DRG) neurons in response to a variety of cytotoxic stimuli. Caspase-2 processing does not occur in thymocytes lacking Apaf-1 or caspase-9, suggesting that in this cell type, activation of caspase-2 occurs downstream of apoptosome formation.

authors

  • O'Reilly, LA
  • Ekert, P
  • Harvey, N
  • Marsden, V
  • Cullen, L
  • Vaux, DL
  • Hacker, G
  • Magnusson, C
  • Pakusch, M
  • Cecconi, F
  • Kuida, K
  • Strasser, A
  • Huang, DCS
  • Kumar, S

publication date

  • August 2002

has subject area