Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. Academic Article uri icon


  • Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.


  • Lötvall, J
  • Hill, AF
  • Hochberg, F
  • Buzás, EI
  • Di Vizio, D
  • Gardiner, C
  • Gho, YS
  • Kurochkin, IV
  • Mathivanan, S
  • Quesenberry, P
  • Sahoo, S
  • Tahara, H
  • Wauben, MH
  • Witwer, KW
  • Théry, C

publication date

  • 2014