Chemically skinned single fibers from adult rat skeletal muscles were used to test the hypothesis that, in mammalian muscle fibers, myosin heavy chain (MHC) isoform expression and Ca2+- or Sr2+-activation characteristics are only partly correlated. The fibers were first activated in Ca2+- or Sr2+-buffered solutions under near-physiological conditions, and then their MHC isoform composition was determined electrophoretically. Fibers expressing only the MHC I isoform could be appropriately identified on the basis of either the Ca2+- or Sr2+-activation characteristics or the MHC isoform composition. Fibers expressing one or a combination of fast MHC isoforms displayed no significant differences in their Ca2+- or Sr2+-activation properties; therefore, their MHC isoform composition could not be predicted from their Ca2+- or Sr2+-activation characteristics. A large proportion of fibers expressing both fast- and slow-twitch MHC isoforms displayed Ca2+- or Sr2+-activation properties that were not consistent with their MHC isoform composition; thus both fiber-typing methods were needed to fully characterize such fibers. These data show that, in rat skeletal muscles, the extent of correlation between MHC isoform expression and Ca2+- or Sr2+-activation characteristics is fiber-type dependent.