Efficient discovery of immune response targets by cyclical refinement of QSAR models of peptide binding Academic Article uri icon

abstract

  • Peptides that induce and recall T-cell responses are called T-cell epitopes. T-cell epitopes may be useful in a subunit vaccine against malaria. Computer models that simulate peptide binding to MHC are useful for selecting candidate T-cell epitopes since they minimize the number of experiments required for their identification. We applied a combination of computational and immunological strategies to select candidate T-cell epitopes. A total of 86 experimental binding assays were performed in three rounds of identification of HLA-A11 binding peptides from the six preerythrocytic malaria antigens. Thirty-six peptides were experimentally confirmed as binders. We show that the cyclical refinement of the ANN models results in a significant improvement of the efficiency of identifying potential T-cell epitopes.

authors

  • Brusic, Vladimir
  • Bucci, Kim
  • Schönbach, Christian
  • Petrovsky, Nikolai
  • Zeleznikow, John
  • Kazura, James W

publication date

  • October 2001