Multiharmonic-generation biopsy of skin Academic Article uri icon


  • Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200-1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.


  • Sun, Chi-Kuang
  • Chen, Cheng-Chi
  • Chu, Shi-Wei
  • Tsai, Tsung-Han
  • Chen, Yung-Chih
  • Lin, Bai-Ling

publication date

  • December 15, 2003