The aim of this study was to determine if anterior cruciate ligament reconstructed (ACL-R) female athletes exhibit altered lower limb kinematic profiles during jump landing when compared to a non-injured age, sex, and activity matched control group. Fourteen ACL-R and 14 non-injured control subjects performed 3 vertical drop jump (DVJ) trials. Lower limb kinematics were recorded at 200 Hz. Peak and time-averaged angular displacements were quantified and utilized for between-group analysis. The ACL-R group displayed altered hip joint frontal and transverse plane kinematic alterations, and knee joint frontal and sagittal plane kinematic alterations. Specifically the ACL-R group displayed an increased adducted (p < 0.05) and internally rotated (p < 0.05) hip joint position, both peak and time-averaged, following landing. The ACL-R group also displayed a decreased adducted (p < 0.05) and flexed (p < 0.05) position of the knee joint following landing. The observed aberrant lower limb kinematics could pre-dispose ACL-R athletes to potential future knee joint injuries. Further studies are required to determine in a prospective manner whether such deficits increase the incidence of recurrent ACL injury, and whether specific sensorimotor protocols following ACL reconstruction can minimize these kinematic deficits.