The thigh adductor squeeze test is commonly used in the diagnosis of groin injuries. Currently no reports exist in the published literature which, detail the level of activation of the adductor musculature during the test as well as concomitant pressure values. Thus the aim of the present study was to investigate adductor muscle activity and concomitant pressure values during the performance of the thigh adductor squeeze test at 0°, 45°, and 90° of hip flexion. Eighteen Gaelic games athletes without any history of groin injury participated. Each participant performed 3 repetitions of the thigh adductor squeeze test in the three positions of 0°, 45°, and 90° of hip flexion. Adductor musculature surface electromyographic activity (bilateral) and pressure values quantified using a commercially available sphygmomanometer were recorded for each test. The greatest pressure values were observed in the 45° of hip flexion test position (0°: 202.50 ± 57.28 mmHg; 45°: 236.76 ± 47.29 mmHg; 90°: 186.11 ± 44.01 mmHg; P < 0.05). Similarly, the greatest amount of adductor muscle activity was observed in the 45° of hip flexion test position (P < 0.05). The combined results of the present study suggest that the 45° of hip flexion test position is the optimal thigh adductor squeeze test position for eliciting adductor muscle activity and maximum pressure values.