To report psychosocial differences between self-reported HPV-positive and HPV-negative Florida men.Men (n=536) in an HPV natural-history study completed a cross-sectional survey (2007-2010).Most participants were white, non-Hispanic, and unmarried with some college education. HPV knowledge was high overall. HPV-positive men reported more negative emotions and perceived threat (P<0.05). HPV-negative men were more likely to disclose test results. Most men (91%) reported strong intentions for the HPV vaccine if it became available for men.Men reported an array of responses to HPV tests with intentions to receive the HPV vaccine. Findings are important for HPV-related health messages for men.